Monday, January 31, 2011
Thursday, January 27, 2011
Leech Neurophysiology Lab
Purpose: To record the electrical activity in the neurons.
Hypothesis: It will be easy to identify the electrical activity of the leech because the ganglia is large and they will all respond the same.
Materials: feather, probe, forceps, scissors, pins, scalpel, dissection tray, leech tank, 20% ethanol, leech tongs, dissection microscope, micromanipulator, oscilloscope, leech
Procedure: Anesthetize the leach, after stretching the leech, pin the leech on its head and tail, dorsal side up. Remove the insides and remove the ganglion. Cut out the ganglion window and set it aside from the others, then cut the sinus. Probe and identify the ganglion sensory cells using different stimuli.
Result/Conclusions: One ganglion consists of many cells that are stimulated by different or similar stimuli. Since the leech has a small system there are five cell types that can be found. (Cell Types N, T, P, R, and X) Each cell or neuron was found by responding to the feather (weak probing), probe (medium probing), and forceps (strong probing). Some of the cell types responded to all three of the stimuli, but some only responded to a couple stimuli and remained unresponsive. The many responses to a single ganglia portrays the immense amount of stimulation that the entire nervous system of the leech undergoes. Although it is a simple nervous system to work with, it is very complex
Hypothesis: It will be easy to identify the electrical activity of the leech because the ganglia is large and they will all respond the same.
Materials: feather, probe, forceps, scissors, pins, scalpel, dissection tray, leech tank, 20% ethanol, leech tongs, dissection microscope, micromanipulator, oscilloscope, leech
Procedure: Anesthetize the leach, after stretching the leech, pin the leech on its head and tail, dorsal side up. Remove the insides and remove the ganglion. Cut out the ganglion window and set it aside from the others, then cut the sinus. Probe and identify the ganglion sensory cells using different stimuli.
Result/Conclusions: One ganglion consists of many cells that are stimulated by different or similar stimuli. Since the leech has a small system there are five cell types that can be found. (Cell Types N, T, P, R, and X) Each cell or neuron was found by responding to the feather (weak probing), probe (medium probing), and forceps (strong probing). Some of the cell types responded to all three of the stimuli, but some only responded to a couple stimuli and remained unresponsive. The many responses to a single ganglia portrays the immense amount of stimulation that the entire nervous system of the leech undergoes. Although it is a simple nervous system to work with, it is very complex
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